Phenotypic and genotypic methods for typing Campylobacter jejuni and Campylobacter coli in poultry
نویسندگان
چکیده
منابع مشابه
Phenotypic and genotypic methods for typing Campylobacter jejuni and Campylobacter coli in poultry.
Human campylobacteriosis, an infection caused by the bacterium Campylobacter, is a major issue in the United States food system, especially for poultry products. According to the Center for Disease Control, campylobacterosis is estimated to affect over 2.4 million people annually. Campylobacter jejuni and Campylobacter coli are 2 species responsible for the majority of campylobacterosis infecti...
متن کاملEvaluation of phenotypic and genotypic methods for subtyping Campylobacter jejuni isolates from humans, poultry, and cattle.
Six methods for subtyping of Campylobacter jejuni were compared and evaluated with a collection of 90 isolates from poultry, cattle, and sporadic human clinical cases as well as from a waterborne outbreak. The applied methods were Penner heat-stable serotyping; automated ribotyping (RiboPrinting); random amplified polymorphic DNA typing (RAPD); pulsed-field gel electrophoresis (PFGE); restricti...
متن کاملMolecular typing of Campylobacter jejuni and Campylobacter coli.
Shi et al. (7) presented data on the development and application of a PCR-based restriction fragment length polymorphism analysis of a 9.6-kb portion of the lipopolysaccharide gene (LG) cluster of Campylobacter jejuni and Campylobacter coli. This proposed molecular subtyping system is novel and has the potential of being directly applicable to epidemiology, prevalence, and other studies. I agre...
متن کاملPrevalence of Campylobacter jejuni and Campylobacter coli in captive wildlife species of India
Campylobacteriosis is an important zoonotic disease and the prevalence of Campylobacter is largely unknown in the wildlife of India. A total of 370 samples, comprising of 314 fresh faecal samples from apparently healthy captive wild animals and birds, 30 stool swabs from animal care takers and 26 samples of the animals’ food and water were collected from G. B. Pant High Altitude Zoo, Nainital, ...
متن کاملPCR-ELISAs for the detection of Campylobacter jejuni and Campylobacter coli in poultry samples.
Campylobacter species, primarily Campylobacter jejuni and Campylobacter coli, are regarded as a major cause of human gastrointestinal disease, commonly acquired by eating undercooked chicken. We describe a PCR-ELISA for the detection of Campylobacter species and the discrimination of C. jejuni and C. coli in poultry samples. The PCR assay targets the 16S/23S ribosomal RNA intergenic spacer regi...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2012
ISSN: 0032-5791
DOI: 10.3382/ps.2011-01414